Epithelial cells sexual assault

JavaScript is disabled for your browser. Some features of this site may not work without it. Optimization of enzymatic lysis of epithelial cells for application to differential extraction of forensic sexual assault samples. Date Issued
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Differential extraction

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Differential extraction - Wikipedia

In sexual-assault cases, autosomal DNA analysis of gynecological swabs is a challenge, as the presence of a large quantity of female material may prevent detection of the male DNA. A solution to this problem is differential DNA extraction, but there is no established best practice for this. We decided to test the efficacy of a number of different protocols on simulated casework samples. Four difficult samples were sent to the nine Swiss laboratories active in forensic genetics.
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Differential extraction also known as differential lysis refers to the process by which the DNA from two different types of cells can be extracted without mixing their contents. The most common application of this method is the extraction of DNA from vaginal epithelial cells and sperm cells from sexual assault cases in order to determine the DNA profiles of the victim and the perpetrator. Its success is based on the fact that sperm cells pack their DNA using protamines rather than histones which are held together by disulfide bonds. The epithelial DNA in solution is removed and saved, while the sperm cell's DNA precipitates with the attached protamines. Differential extraction uses a chemical called dithiothreitol DTT to disrupt the sulfur bonds in the protamines in order to release its DNA.
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